Artikelnummer GC-047-1000
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BioTherm™ Mix

BESCHREIBUNG


BioThermMix™ ist ein 2.5x konzentrierter Reagenzien-Mix für PCR-Reaktionen.

Der Mix enthält Bio-Therm™ DNA-Polymerase (0.06 u/μl), 2.5x PCRPuffermit 3.75 mM MgCl2, 500 μM von jedem dNTP
und stabilisierende Substanzen. Die 2.5x Konzentrationdes BioThermMix™ ermöglicht einen flexiblen
Einsatz in den PCR-Reaktionen. Auf diese Weise kannbis zu 60 % des endgültigen Reaktion-Volumens für
die Zugabe von Primern, Template-DNA, sowie anderen Reaktionssubstanzen genutzt werden.

APPLICATION

BioTherm™ Mix is suitable and tested for amplification of genomic targets ranging from l00 bp to 4 kb and of episomal targets (lambda phage; plasmids) up to 10 kb under various reaction conditions.

  • high through-put PCR
  • routine diagnostic PCR requiring high reproducibility
  • DNA sequencing template preparation


STORAGE CONDITION

BioTherm™ Mix can be stored at either -20°C in a freezer or at 2-8°C in a usual refrigerator. Shipment at ambient temperature is possible without reduction of PCR performance and activity. Storage at 2-8°C is convenient for easy and time-saving assembling of the PCR assays. Storage of BioTherm™ Mix at -20°C is recommended for long-term storage after the mix has been sed once under non-sterile conditions. Multiple free-zing and thawing do not affect the performance or activity of the Mix.

At 2-8°C the BioTherm™ Mix is at least stable for 12 months, in frozen state for 2 years.


IMPORTANT NOTE

The stabilizers in the BioTherm™ Mix are potential growth substrates for bacterial contaminations! If the Mix has been opened and used under non-sterile conditions, the residual moiety should be used during the next 2 weeks with intermediate storage at 2-8°C or should be frozen at -20°C for longer storage!


NOTE

Do not contaminate the BioTherm™ Mix with primers and template DNA used in individual reactions. Thaw and mix all components thoroughly, spin down shortly and chill on ice.
It is very important to mix the BioTherm™ Mix before use to avoid localized concentration!


PROTOCOL

    1. Place the PCR tubes on ice.

    1. Prepare first a template/primer mix according to the volumes given in the
      table below for different reaction volumes. Mix the template/primer mix and
      chill on ice.

    1. Dispense now the corresponding volume of BioTherm™ Mix (20 µl for a 50
      µl reaction) followed by the tem-plate/primer mix into each reaction tube.
      Close tubes and mix well. Spin down shortly, if necessary, and chill the tubes
      on ice.

    1. Start the program on the thermal cycler. Transfer the tubes directly from
      ice into the thermal cycler when the temperature of the block has reached
      90°C.


REACTION VOLUME


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