SKU GC-067-100
Manufacturer part number
Availability

2x SYBR ® Green I Hot-Start real-time PCR-Mix

Recombinant Q-Therm™ Taq DNA polymerase is a variant of Taq DNA polymerase with a genetically modified pol gene providing a Hot-Start for PCR.
90,00 € excl tax

DESCRIPTION

2x SYBR® Green I Hot-Start real-time PCR-Mix is a 2x-concentrated, ready-to-use reaction cocktail containing all components, except primers, for the amplification and detection of DNA in real-time quantitative PCR with intercalating dye SYBR® Green I.
Recombinant Q-Therm™ Taq DNA polymerase is a variant of Taq DNA polymerase with a genetically modified pol gene providing a Hot-Start for PCR. The modification renders the enzyme inactive at 37°C. At temperatures over 68°C the enzyme restores its activity. Pre-heating at 95°C is not needed to activate this enzyme.


CONCENTRATION

2x


STORAGE TEMPERATURE

Store 2x SYBR® Green I Hot-Start real-time PCR-Mix at -15 to -25°C. For short-term storage the master mix may be stored at +2 to +8°C. Keep the master mix away from light. Avoid repeated freezing and thawing.


2X PCR MIX COMPOSITION

Q-Therm™ Taq DNA polymerase 0.05 units/µl
20 mM Tris-HCl pH 8.8 (at 25ºC)
110 mM KCl
0.05% Tween-20
5 mM MgCl2
dNTPs 0.4 mM of each
SYBR® Green I


PACK SIZE

2x 1.25 ml (for 100 reactions in 50 µl assay)


For 25 µl real-time PCR reaction add the following components:


Amplification protocol (for Bio-Rad iCycler):


An example of the quantitative PCR with human PII gene primers and the standard curve construction:


Amplification protocol (Bio-Rad iCycler):


PCR amplification curve:


Melting curve:


Standard curve:

Free Sample verfügbar? Ja

Product tags

SYBR (1)