DESCRIPTION
GeneCraft offers most restriction enzymes from pBS polylinker. Reaction buffers are supplied together with enzymes free of charge.
UNIT DEFINITION & CONCENTRATION
One unit of the enzyme is the amount required to hydrolyze 1 µg of DNA hour in a total reaction volume of 50nml. Most restriction enzymes are normallly delivered at the concentration of 20 units/µl.
STORAGE BUFFER
20 mM Tris- HCL pH 7.5; 1000mM NaCl; 0.1 mM EDTA; 2 mM DTT; 0.1 mg/ml BSA; 50% glycerol (v/v)
STORAGE TEMPERATURE
Store restriction rnzymes below 0°C, preferably at - 20°C, in a constant temperature freezer.
Schnittmuster
5' ---> 3'
3' ---> 5' |
Activity [%] in buffer |
Optimal reaction
temp. |
Heat inactivable
20' at |
| A |
B |
L |
M |
H |
R/AATTY
YTTAA/R |
0 - 25 |
100 |
25 - 50 |
50 - 75 |
50 - 75 |
50°C |
|
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