SKU GC-RE-017
Availability

Acs I

175,00 € excl tax

DESCRIPTION

GeneCraft offers most restriction enzymes from pBS polylinker. Reaction buffers are supplied together with enzymes free of charge.

UNIT DEFINITION & CONCENTRATION

One unit of the enzyme is the amount required to hydrolyze 1 µg of DNA hour in a total reaction volume of 50nml. Most restriction enzymes are normallly delivered at the concentration of 20 units/µl.

STORAGE BUFFER

20 mM Tris- HCL pH 7.5; 1000mM NaCl; 0.1 mM EDTA; 2 mM DTT; 0.1 mg/ml BSA; 50% glycerol (v/v)

STORAGE TEMPERATURE

Store restriction rnzymes below 0°C, preferably at - 20°C, in a constant temperature freezer.

Schnittmuster
5' ---> 3'
3' ---> 5'
Activity [%] in buffer Optimal reaction
temp.
Heat inactivable
20' at
A B L M H
R/AATTY
YTTAA/R
0 - 25 100 25 - 50 50 - 75 50 - 75 50°C  

==