DESCRIPTION
BioThermAB HotStart Taq DNA polymerase offers all the benefits of BioTherm DNA polymerase plus an antibody-based, built-in hot start. The BioThermAB polymerase mix contains HotStart Taq Monoclonal Antibody, which blocks polymerase activity prior to the onset of thermal cycling. This prevents primer-dimers and other artifacts resulting from low-level synthesis from nonspecifically primed sites. The antibodies are quickly inactivated by the increased temperature of thermal cycling. BioThermAB polymerase requires no prolonged heating or denaturing step as do other hot-start methods, making BioThermAB polymerase more convenient and easy to use.
APPLICATION
- Hot-Start-PCR
- PCR with high specificity
CONCENTRATION
5 units/µl
UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble formcin 30 min at 72°C.
STORAGE TEMPERATURE
-20°C
10X REACTION BUFER
160 mM (NH4)2SO4, 670 mM Tris-HCl pH 8.8 (at 25°C), 15 mM MgCl2, 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.
QUALITY CONTROL
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases
STORAGE BUFFER
10mM K-phosphate buffer pH 7.0, 100mM NaCl, 0.5mM EDTA, 1mM DTT, 0.01% Tween 20; 50% glycerol (v/v)
