BioThermPlus™ DNA Polymerase is designed for maximum PCR success. It is a blend of BioTherm™ DNA Polymerase and Accutherm™ DNA Polymerase which allows amplification of longer templates with greater success and higher yields than either enzyme component alone. Together with a optimized buffer BioThermPlus™ DNA Polymerase provides the highest success rate of any PCR enzyme or enzyme blend. The enzyme formulation produces high PCR product yield from a wide variety of templates up to 10 kb. It´s also provides superior sensitivity by amplifying samples where starting material is limited.
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form in 30 minutes at 72ºC under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic acid, sodium salt) pH 9.3 (at 25ºC); 50 mM KCl; 2 mM MgCl2; 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol(v/v)
Store BioTherm™ DNA polymerase below 0ºC, preferably at -20ºC, in a constant temperature freezer.
10X REACTION BUFER
160 mM (NH 4 ) 2 SO 4 , 670 mM Tris-HCl pH 8.8 (at 25ºC), 15 mM MgCl 2 , 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.
1.5 ml 10x reaction buffer (contains 15 mM MgCl2) Cat. No. GC-002-006
1.5 ml 10x reaction buffer without MgCl2 plus 50 mM MgCl2 separately Cat. No. GC-002-007
1 Kaledin, A.S., et al. (1980) Biokhimiya 45, 494
2 Kaledin, A.S., et al. (1981) Biokhimiya 45, 1576
3 Kaledin, A.S., et al. (1982) Biokhimiya 47, 1785