BioThermRed™ DNA polymerase is a mixture of BioTherm™ DNA polymerase and red pigment that works like BioTherm™. After addition of BioThermRed™ a thin red layer on the bottom of the tube appears. So you have a visible pipetting control. Furthermore you can see, if your reaction is already mixed, when the color of your reaction becomes uniform.
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form
in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic
acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20,0.2% indicator red dye, 50% glycerol (v/v) The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.
Store BioThermRed™ DNA Polymerase below 0ºC, preferably at -20°C, in a constant temperature freezer.
10X REACTION BUFER
160 mM (NH4)2SO4, 670 mM Tris-HCl pH 8.8 (at 25°C), 15 mM MgCl2, 0.1% Tween 20
1.5 ml 10x reaction buffer (contains 15 mM MgCl2) Cat. No. GC-002-006
1.5 ml 10x reaction buffer without MgCl2 plus 50 mM MgCl2 separately Cat. No. GC-002-007
Endonuclease- and exonuclease activities were not detectable after 2 and 1 hours incubation, respectively, of
1 µg lambda DNA and 0.22 µg of EcoRI digested lambda DNA, respectively, at 72°C in the presence of 15-
20 units of BioThermRed™ DNA Polymerase.
BioTherm™ DNA Polymerase, SupraTherm™ DNA Polymerase, dNTPs