DESCRIPTION
BioThermRed™ DNA polymerase is a mixture of BioTherm™ DNA polymerase and red pigment that works like BioTherm™. After addition of BioThermRed™ a thin red layer on the bottom of the tube appears. So you have a visible pipetting control. Furthermore you can see, if your reaction is already mixed, when the color of your reaction becomes uniform.
CONCENTRATION
5 units/µl
UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form
in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic
acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.
STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20,0.2% indicator red dye, 50% glycerol (v/v) The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.
STORAGE TEMPERATURE
Store BioThermRed™ DNA Polymerase below 0ºC, preferably at -20°C, in a constant temperature freezer.
10X REACTION BUFER
160 mM (NH4)2SO4, 670 mM Tris-HCl pH 8.8 (at 25°C), 15 mM MgCl2, 0.1% Tween 20
1.5 ml 10x reaction buffer (contains 15 mM MgCl2) Cat. No. GC-002-006
1.5 ml 10x reaction buffer without MgCl2 plus 50 mM MgCl2 separately Cat. No. GC-002-007
ASSOCIATED ACTIVITIES
Endonuclease- and exonuclease activities were not detectable after 2 and 1 hours incubation, respectively, of
1 µg lambda DNA and 0.22 µg of EcoRI digested lambda DNA, respectively, at 72°C in the presence of 15-
20 units of BioThermRed™ DNA Polymerase.
COMPANION PRODUCTS
BioTherm™ DNA Polymerase, SupraTherm™ DNA Polymerase, dNTPs
