BioThermStar™ Hot Start Taq DNA Polymerase

Thermostable DNA polymerase purified from the Thermus aquaticus strain in accordance with the procedures developed by Kaledin for the isolation of thermostable enzymes processing DNA polymerase activity from thermophilic bacteria. BioThermStar™ is a modified form of the enzyme designed for Hot-Start-PCR.
Produkt Artikel-Nr. Packungsgröße Preise
BioThermStar™ Hot Start Taq DNA Polymerase
GC-045-0250
Availability:
250 u
32,50 € excl tax
BioThermStar™ Hot Start Taq DNA Polymerase
GC-045-0500
Availability:
500 u
65,00 € excl tax
BioThermStar™ Hot Start Taq DNA Polymerase
GC-045-1000
Availability:
1000 u
130,00 € excl tax
BioThermStar™ Hot Start Taq DNA Polymerase
GC-045-5000
Availability:
5000 u
650,00 € excl tax
BioThermStar™ Hot Start Taq DNA Polymerase
GC-045-0100
Availability:
100 u
13,00 € excl tax

DESCRIPTION

BioThermStar™ is a thermostable DNA polymerase purified from the Thermus aquaticus strain in accordance with the procedures developed by Kaledin for the isolation of thermostable enzymes processing DNA polymerase activity from thermophilic bacteria. BioThermStar™ is a modified form of the enzyme designed for Hot-Start-PCR. It is a highly processive 5'-3' DNA polymerase lacking 3'-5'-exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- or exonucleases.


BUFFER pH

We strongly recommend to use buffers at pH 8.3 with a concentration of 1,5mM MgCl2!


ACTIVATION

This polymerase is inactive until incubated 7-10 min at 95°C!!! These activation conditions are extremely important!!! The activation completely prevents nonspecific primer annealing and the formation of primer-dimers during setup.


REACTION

It is also very important to pay great attention to the actual temperature (95°C) inside the tube!!!


APPLICATION

Hot-Start-PCR
PCR with high specificity


CONCENTRATION

5 units/µl


UNIT DEFINITION

One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 min at 72°C.


STORAGE BUFFER

10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol (v/v)


STORAGE TEMPERATURE

-20°C


10X REACTION BUFER

160 mM (NH 4 ) 2 SO 4 , 670 mM Tris-HCl pH 8.3 (at 25°C), 15 mM MgCl 2 , 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.
Please note the difference between BioTherm ™ and BioThermStar ™ buffers!


QUALITY CONTROL

Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.


COMPANION PRODUCTS

BioThermAB™, MagicTubes™


BioThermStar IN A TAQMAN ASSAY

Free Sample verfügbar? Ja

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