BioThermStar™ is a thermostable DNA polymerase purified from the Thermus aquaticus strain in accordance with the procedures developed by Kaledin for the isolation of thermostable enzymes processing DNA polymerase activity from thermophilic bacteria. BioThermStar™ is a modified form of the enzyme designed for Hot-Start-PCR. It is a highly processive 5'-3' DNA polymerase lacking 3'-5'-exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- or exonucleases.
We strongly recommend to use buffers at pH 8.3 with a concentration of 1,5mM MgCl2!
This polymerase is inactive until incubated 7-10 min at 95°C!!! These activation conditions are extremely important!!! The activation completely prevents nonspecific primer annealing and the formation of primer-dimers during setup.
It is also very important to pay great attention to the actual temperature (95°C) inside the tube!!!
PCR with high specificity
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 min at 72°C.
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol (v/v)
10X REACTION BUFER
160 mM (NH 4 ) 2 SO 4 , 670 mM Tris-HCl pH 8.3 (at 25°C), 15 mM MgCl 2 , 0.1% Tween 20
The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.
Please note the difference between BioTherm ™ and BioThermStar ™ buffers!
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
BioThermStar IN A TAQMAN ASSAY