DESCRIPTION
BioThermT™ is a modified BioTherm™ DNA Polymerase to facilitate incorporation of Biotin- and Digoxigenin-dUTP in DNA.
BESCHREIBUNG
BioThermT™ ist eine modifizierte BioTherm™ DNA Polymerase, die wesentlich effizienter als herkömmliche Taq Polymerase Biotin- und Digoxigenin-dUTP in DNA einbringt.
CONCENTRATION
5 units/µl
UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM ß-mercaptoethanol) and activated free of charge.
STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol (v/v)
STORAGE TEMPERATURE
Store BioThermT™ DNA Polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.
10X REACTION BUFER
160 mM (NH4)2SO4, 670 mM Tris-HCl pH 8.8 (at 25°C), 15 mM MgCl2, 0.1% Tween 20
Cat.No. GC-002-006
1.5 ml 10x reaction Buffer (contains MgCl2)
Cat.No. GC-002-007
1.5 ml 10x reaction buffer without MgCl2 plus 50mM MgCl2 separately
ASSOCIATED ACTIVITIES
Endonuclease- and Exonuclease activities were not detectable after 2 and 1 hours incubation, respectively, of 1 µg lambda DNA and 0.22 µg of EcoRI digested lambda DNA, respectively, at 72°C in the presence of 15-20 units BioThermT™ DNA Polymerase.
COMPANION PRODUCTS
BioTherm™, BioThermBio™
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