Klenow fragment

Klenow fragment is a DNA-dependent polymerase with 3'-5' exonuclease activity.
Produkt Artikel-Nr. Packungsgröße Preise
Klenow fragment
GC-009-0100
Availability:
100 u
12,00 € excl tax
Klenow fragment
GC-009-0250
Availability:
250 u
31,25 € excl tax
Klenow fragment
GC-009-0500
Availability:
500 u
62,50 € excl tax
Klenow fragment
GC-009-1000
Availability:
1000 u
125,00 € excl tax
Klenow fragment
GC-009-5000
Availability:
5000 u
625,00 € excl tax

DESCRIPTION

Klenow fragment is a DNA-dependent polymerase with 3'-5' exonuclease activity. It lacks 5'-3' exonuclease activity of the native enzyme and is suited for random-primed labelling of DNA with random oligonucleotides and for filling-in of 5' protruding ends to blunt-ends.


CONCENTRATION

5 units/µl


UNIT DEFINITION

One unit is the amount of enzyme that incorporates 10 nmoles of deoxynucleotides into acid-insoluble form in 30 minutes at 37°C.


STORAGE BUFFER

20 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 2 mM DTT, 0.1 mg/ml BSA; 50% glycerol (v/v)


STORAGE TEMPERATURE

Store Klenow Enzyme below 0ºC, preferably at -20ºC, in a constant temperature freezer.


10X REACTION BUFER

100 mM Tris-HCl pH 7.5, 100 mM MgCl2 , 10 mM DTE
(= buffer L for restriction endonucleases!)


COMPANION PRODUCTS

T4 DNA Ligase, dNTP

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