RNase-Inhibitor

Produkt Artikel-Nr. Packungsgröße Preise
RNase-Inhibitor
GC-042-0100
Availability:
100 u
5,00 € excl tax
RNase-Inhibitor
GC-042-0250
Availability:
250 u
12,50 € excl tax
RNase-Inhibitor
GC-042-0500
Availability:
500 u
25,00 € excl tax
RNase-Inhibitor
GC-042-1000
Availability:
1000 u
50,00 € excl tax
RNase-Inhibitor
GC-042-5000
Availability:
5000 u
250,00 € excl tax

DESCRIPTION

Native RNase-Inhibitor from human placenta exerts ist inhibitory effect by binding non-covalently to RNases in a 1:1 ratio with an association constant of 1014. It is a protein with molecular weight of 51 kDa and inhi-bits common eukaryotic RNases including RNase A, RNase B, RNase C.
It does not inhibit RNase H, S1 Nuclease, SP6, T7 or T3 RNA polymerase, AMV or M-MLV Reverse Transcrip-tase, Taq DNA polymerase and RNase T1.
The enzyme is active over a broad pH range between 5 and 8, with a maximum activity at pH 7-8.


APPLICATION

Any application where eukaryotic RNase contamination is a potential problem

  • RNA transcription
  • In vitro RNA translation
  • cDNA synthesis
  • DNA and RNA sequencing
  • RT-PCR


CONCENTRATION

5 units/µl


UNIT DEFINITION

One unit inhibits 5 ng of RNase A by 50% using cytidine 2',3'-cyclic monophosphate (cCMP) as a substrat.


STORAGE BUFFER

20 mM Hepes-KOH, 50 mM KCl, 8 mM DTT, 50% glycerol


STORAGE TEMPERATURE

-20°C


QUALITY CONTROL

Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.


REFERENCES

  1. BLACKBURN, P. (1979) J. Biol. Chem. 254:12484

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