SD Polymerase New Product
SD Polymerase is a novel artificial thermostable polymerase, providing a strong
stranddisplacement activity. Unlike natural enzymes with strong strand displacement activity as Phi29 or Bst–polymerase, which are active below 68 °C only, SD Polymerase is stable up to 93 °C. This allows running isothermal amplification like LAMP with initial heating-up for increased specificity. PCR with SD Polymerase is effective on long template, with extremely low concentrations of template and amplification of difficult GCrichDNA regions. SD Polymerase can be effectively used in PCDR, a combination of PCR and strand displacement ..
SD DNA polymerase has 5’-‐3’ polymerase and 5’-‐3’ strand displacement activities. It does not have any exonuclease activity.
VSD DNA polymerase is a thermostable enzyme. It can be used at temperature up to 93 C. For denaturation step of LAMP or PCR, 92 C is strongly recommended.
conventonal buffer for Taq polymerase
10x reaction buffer without Mg2+, pH 8.75
10 U/mkL (recommended for PCR); or 50-.‐100 U/mkL (recommended for LAMP)
Magnesium concentration: 2.5 – 3.0 mM for PCR; 3-4 mM for LAMP
Temperature optimum is 62 – 70 C
LAMP: Strand Displacement activity of SD polymerase is temperature depended and increases with the temperature. Optimum for LAMP amplification is 62-.‐68 C. High thermostability of the enzyme allows carrying out LAMP with a DNA denaturation step
(92C for 2 min). LAMP can be performed with 40 – 100 units of SD polymerase per 50 mkL.
PCR: SD polymerase is suitable for amplification of short (from 100 bp) and long (up to20-30 kb) DNA fragments from simple (plasmids) and complex (GC-richDNA) templates without special optmisation. In PCR applications SD polymerase demonstrates higher yields, speed and efficiency compared with Taq.
We recommend 92 C for denaturation steps and 68 C for elongation steps of PCR.
We recommend using 2.5 - 20 units of SD polymerase per 50 mkL for PCR.
Elongation time: 15 – 40 sec/kb
For Hot Start PCR SD polymerase can be used with anti’Taq MAbs