SD Polymerase neues Produkt

DESCRIPTION

SD ™ Polymerase is a novel artificial thermostable polymerase, providing a strong
stranddisplacement activity. Unlike natural enzymes with strong strand displacement activity as Phi29 or     Bst–polymerase, which are active below 68 °C only, SD™ Polymerase is stable up to 93 °C. This allows running isothermal amplification like LAMP with initial heating-up for increased specificity. PCR with SD™ Polymerase is effective on long template, with extremely low concentrations of template and amplification of difficult GCrichDNA regions. SD ™ Polymerase can be effectively used in PCDR, a combination of PCR and strand displacement ..

APPLICATIONS

SD™ DNA polymerase has 5’-­‐3’ polymerase and 5’-­‐3’ strand displacement activities. It does not have any exonuclease activity.

STORAGE TEMPERATURE

SD™ DNA polymerase is a thermostable enzyme. It can be used at temperature up to 93 C. For denaturation step of LAMP or PCR, 92 C is strongly recommended.


STORAGE BUFFER

conventonal buffer for Taq polymerase

REACTION BUFFER

10x reaction buffer without Mg2+, pH 8.75

CONCENTRATION

10 U/mkL (recommended for PCR); or 50-.‐100 U/mkL (recommended for LAMP)

OPTIMAL CONCENTRATION

Magnesium concentration: 2.5 – 3.0 mM for PCR; 3-4 mM for LAMP

Temperature optimum is 62 – 70 C

LAMP: Strand Displacement activity of SD™ polymerase is temperature depended and increases with the temperature. Optimum for LAMP amplification is 62-.‐68 C. High thermostability of the enzyme allows carrying out LAMP with a DNA denaturation step
(92C for 2 min). LAMP can be performed with 40 – 100 units of SD polymerase per 50 mkL.

PCR: SD™ polymerase is suitable for amplification of short (from 100 bp) and long (up to20-30 kb) DNA fragments from simple (plasmids) and complex (GC-richDNA) templates without special optmisation. In PCR applications SD™ polymerase demonstrates higher yields, speed and efficiency compared with Taq.

We recommend 92 C for denaturation steps and 68 C for elongation steps of PCR.

We recommend using 2.5 - 20 units of SD ™ polymerase per 50 mkL for PCR.
Elongation time: 15 – 40 sec/kb


For Hot Start PCR SD™ polymerase can be used with anti’Taq  MAbs