SP6 RNA Polymerase

Isolated from a recombinant E. coli strain this DNA-dependent RNA polymerase is strictly specific for its promoter.
Produkt Artikel-Nr. Packungsgröße Preise
SP6 RNA Polymerase
GC-012-1000
Manufacturer part number:
Availability:
1000 u
45,00 € excl tax
SP6 RNA Polymerase
GC-012-10000
Manufacturer part number:
Availability:
10000 u
450,00 € excl tax

DESCRIPTION

Isolated from a recombinant E. coli strain this DNA-dependent RNA polymerase is strictly specific for its promoter. It is used to transcribe RNA from DNA templates, cloned into vectors containing SP6 promoter.


CONCENTRATION

60 units/µl


UNIT DEFINITION

One unit is the amount of enzyme that incorporates 1 nmol of labelled nucleoside triphosphates into acid-precipitable RNA in 60 minutes at 37°C.


STORAGE BUFFER

20 mM Tris-HCl pH 7.5; 100 mM NaCl; 0,1 mM EDTA; 2 mM DTT; 0,1 mg/ml BSA; 50% glycerol (v/v)


STORAGE TEMPERATURE

Store SP6 RNA polymerase below 0ºC, preferably at -20ºC, in a constant temperature freezer.


5X REACTION BUFFER

200 mM Tris-HCl pH 7.9; 30 mM MgCl2; 50 mM DTT