DESCRIPTION
SupraTherm™ Taq DNA Polymerase is a thermostable Taq DNA polymerase purified from the Thermus aquaticus strain by several rounds of liquid chromatography. The purity of SupraTherm™ Taq DNA Polymerase is more then 90% of the total protein in the preparation. Amplification of DNA fragments (100 bp to 5 kb) can be achieved with it. The enzyme has both, 5'-3' polymerase- and 5'-3'exonuclease activities. SupraTherm™ can add a single template-directed deoxyadenosin (A) residue to the 3’ end of duplex PCR products. This property allows easy and efficient ligation of PCR products in TA cloning vectors.
APPLICATION
The enzyme may be used for PCR, thermosequencing and for other research and diagnostic applications.
SupraTherm™ DNA Polymerase is the best enzyme for everyday routine applications. The enzyme has limited
application for PCR with low-abundance template (less then 100 DNA molecules of template). Please use for
PCR of low-abundance templates and for other more sophisticated techniques BioTherm™ DNA Polymerase.
CONCENTRATION
5 units/µl
UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-propa-nesulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2 , 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.
STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20;
50% glycerol (v/v)
STORAGE TEMPERATURE
Store SupraTherm™ DNA Polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.
10X REACTION BUFER
160 mM (NH4)2SO4, 670 mM Tris-HCl pH 8.8 (at 25ºC), 15 mM MgCl2, 0.1% Tween 20
The 10x reaction buffer(on request with or without MgCl2) is delivered free of charge.
1.5 ml 10x reaction buffer (contains 15 mM MgCl 2 ) Cat. No. GC-002-006
1.5 ml 10x reaction buffer with MgCl2 plus 50 mM MgCl2 separately Cat. No. GC-002-007
QUALITY CONTROL
SupraTherm™ DNA Polymerase was tested for the absence of unspecific endo- and exonucIeases activities.
COMPANION PRODUCTS
BiothermRed™ DNA Polymerase, BioTherm™ DNA Polymerase, dNTPs, T-Vector
