BESCHREIBUNG
SupraTherm™ DNA-Polymerase ist eine hitzestabile DNA-Polymerase, die in mehreren Durchläufen mittels Flüssigkeits-Chromatographie aus Thermus aquaticus isoliert wurde. Der Reinheitsgrad der SupraTherm™ DNA-Polymerase beträgt mehr als 90 % bezogen auf den Gesamt-Proteingehalt. Das Enzym ist in der Lage DNA-Fragmente mit einer Länge von 100 bp bis 5 kb zu amplifizieren. SupraTherm™ DNA-Polymerase besitzt sowohl eine 5´-3´ Polymerase- als auch eine 5´-3´ Exonuklease-Aktivität. Das Enzym kann einen einzelnen,Template-abhängigen Deoxyadenosin (A) –Rest an das 3´ -Ende doppelsträngiger PCR-Produkte anhängen.
APPLICATION
The enzyme may be used for PCR, thermosequencing and for other research and diagnostic applications.
SupraTherm™ DNA Polymerase is the best enzyme for everyday routine applications. The enzyme has limited
application for PCR with low-abundance template (less then 100 DNA molecules of template). Please use for
PCR of low-abundance templates and for other more sophisticated techniques BioTherm™ DNA Polymerase.
CONCENTRATION
5 units/µl
UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-propa-nesulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2 , 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.
STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20;
50% glycerol (v/v)
STORAGE TEMPERATURE
Store SupraTherm™ DNA Polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.
10X REACTION BUFER
160 mM (NH4)2SO4, 670 mM Tris-HCl pH 8.8 (at 25ºC), 15 mM MgCl2, 0.1% Tween 20
The 10x reaction buffer(on request with or without MgCl2) is delivered free of charge.
1.5 ml 10x reaction buffer (contains 15 mM MgCl 2 ) Cat. No. GC-002-006
1.5 ml 10x reaction buffer with MgCl2 plus 50 mM MgCl2 separately Cat. No. GC-002-007
QUALITY CONTROL
SupraTherm™ DNA Polymerase was tested for the absence of unspecific endo- and exonucIeases activities.
COMPANION PRODUCTS
BiothermRed™ DNA Polymerase, BioTherm™ DNA Polymerase, dNTPs, T-Vector
