Synergy™ DNA Polymerase

These components are optimized to achieve amplification of 35 kb or 10 kb products from lambda templates or genomic DNA, respectively.
Produkt Artikel-Nr. Packungsgröße Preise
Synergy™ DNA Polymerase
GC-005-0100
Manufacturer part number:
Availability:
100 u
20,00 € excl tax
Synergy™ DNA Polymerase
GC-005-0250
Manufacturer part number:
Availability:
250 u
50,00 € excl tax
Synergy™ DNA Polymerase
GC-005-0500
Manufacturer part number:
Availability:
500 u
100,00 € excl tax
Synergy™ DNA Polymerase
GC-005-1000
Manufacturer part number:
Availability:
1000 u
200,00 € excl tax
Synergy™ DNA Polymerase
GC-005-5000
Manufacturer part number:
Availability:
5000 u
1.000,00 € excl tax

DESCRIPTION

Synergy is a mix of thermostable components possessing 5'-3' DNA polymerase activity (KlenTherm™ ) and a blend of enzymes (patent pending) improving the accuracy and processivity. These components are optimized to achieve amplification of 35 kb or 10 kb products from lambda templates or genomic DNA, respectively.


CONCENTRATION

10 units/µl


UNIT DEFINITION

One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2 , 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.


STORAGE BUFFER

10 mM K-phosphate buffer pH 7,0; 100 mM NaCI; 0,5 mM EDTA; 1 mM DTT; 0,01% Tween 20; 50% glycerol (v/v)


STORAGE TEMPERATURE

Store Synergy™ DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.


10X REACTION BUFER

160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2

Please note the difference between Synergy™ and BioTherm™ reaction buffers.


COMPANION PRODUCTS

SynergyN™ DNA polymerase, AccuTherm™ DNA polymerase.


TYPICAL REACTION CONDITIONS

Free Sample verfügbar? Ja

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