SynergyN™ is a mix of thermostable polymerases possessing 5'-3' DNA polymerase activity that is optimized to amplify longer GC-rich fragments (KlenThermN™ ) and 3'-5' proof-reading activity (AccuTherm™ ). These components are optimized to achieve amplification of 15 kb products from genomic DNA. A mixture of KlenThermN™ with AccuTherm™ provides more robust synthesis of longer GC-rich amplification products.
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-aminopropane-sulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2, 1 mM .-mercaptoethanol) and activa-ted calf thymus DNA as substrate.
10 mM K-phosphate buffer pH 7.0, 100 mM NaCI, 0.5 mM EDTA; 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v)
Store SynergyN™ DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.
10X REACTION BUFER
160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2
Please note the difference between Synergy™ and BioTherm™ reaction buffers.
Synergy™ DNA polymerase, dNTPs