SynergyPlus™ DNA Polymerase

Taq-Polymerasen mit dem Zusatz einer Pyrophosphatase zur Amplifikation langer Fragmente.
Produkt Artikel-Nr. Packungsgröße Preise
SynergyPlus™ DNA Polymerase
GC-048-0100
Artikelnummer des Herstellers:
Verfügbarkeit:
100 u
30,00 € *
SynergyPlus™ DNA Polymerase
GC-048-0250
Artikelnummer des Herstellers:
Verfügbarkeit:
250 u
75,00 € *
SynergyPlus™ DNA Polymerase
GC-048-0500
Artikelnummer des Herstellers:
Verfügbarkeit:
500 u
150,00 € *
SynergyPlus™ DNA Polymerase
GC-048-1000
Artikelnummer des Herstellers:
Verfügbarkeit:
1000 u
300,00 € *
SynergyPlus™ DNA Polymerase
GC-048-5000
Artikelnummer des Herstellers:
Verfügbarkeit:
5000 u
1.500,00 € *

BESCHREIBUNG

Dies ist eine Mischung von Taq-Polymerasen mit dem  Zusatz einer Pyrophosphatase zur Amplifikation langer  Fragmente. SynergyPlus™ DNA-Polymerase amplifiziert bis zu 20 kb lange Fragmente anhand genomischer DNA.

DESCRIPTION

KlenTherm-based mixture with addition of pyrophosphatase for the amplification of long fragments. SynergyPlus™ DNA polymerase can amplify up to 20 kb from genomic DNA.


APPLICATION

  • Very Long and accurate PCR


CONCENTRATION

5 units/µl


UNIT DEFINITION

One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)methyl-amino-propanesulphonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2 , 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.


STORAGE BUFFER

10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v)


STORAGE TEMPERATURE

Store SynergyPlus™ DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.


REACTION BUFFER

160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2

Please note the difference between Synergy™ and BioTherm™ reaction buffers.


QUALITY CONTROL

Activity, SDS-PAGE purity, absence of endonucleases/ nickases and exonucleases.


REFERENCES

Barnes W.M. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from bacterio-phage templates. Proc. Natl. Acad. Sci. USA 91: 2216-2220