T4 DNA Ligase

E. coli strain and is suitable for ligation of sticky- and blunt ended DNA fragments.
Produkt Artikel-Nr. Packungsgröße Preise
T4 DNA Ligase
GC-011-0100
Availability:
100 u
10,00 € excl tax
T4 DNA Ligase
GC-011-0250
Availability:
250 u
25,00 € excl tax
T4 DNA Ligase
GC-011-0500
Availability:
500 u
50,00 € excl tax
T4 DNA Ligase
GC-011-1000
Availability:
1000 u
100,00 € excl tax
T4 DNA Ligase
GC-011-5000
Availability:
5000 u
500,00 € excl tax

DESCRIPTION

T4 DNA ligase is isolated from a recombinant E. coli strain and is suitable for ligation of sticky- and blunt ended DNA fragments.


CONCENTRATION

10 Weiss units/µl


UNIT DEFINITION

One unit (Weiss unit) is the amount of enzyme that catalyses the conversation of one nanomol 32P-ATP in 20 minutes at 37°C. 1 ligation unit = 0,015 Weiss unit.


STORAGE BUFFER

20 mM Tris-HCI buffer pH 7.5; 100 mM NaCI; 0.1 mM EDTA; 2 mM DTT; 0.1 mg/ml BSA; 50% glycerol (v/v)


STORAGE TEMPERATURE

Store T4 DNA ligase below 0ºC, preferably at -20ºC, in a constant temperature freezer.


10X REACTION BUFER

660 mM Tris-HCI pH 7.5; 50 mM MgCI2; 10 mM DTE; 10 mM ATP
+ extra 1 ml 50% PEG4000 solution on request


REACTION CONDITION

optimal ligation occurs at 15ºC


efficiency of T4 DNA LIGASE


NOTE

PEG (Polyehtylenglycol) greatly increases the rate of ligation of blunt-ended DNA (1). 5% (w/v) is the suggested concentration of PEG4000 in the reaction mixture.


REFERENCES

1. Pheiffer, B.H., Zimmermann, S.B., Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribo-oligonucleotides by T4 DNA Ligase in polymer solutions, Nucleic Acids Res., 11, 7853-7871, 1983

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