T4 polynucleotide kinase

Produkt Artikel-Nr. Packungsgröße Preise
T4 polynucleotide kinase
GC-010-0100
Availability:
100 u
3,50 € excl tax
T4 polynucleotide kinase
GC-010-0250
Availability:
250 u
8,75 € excl tax
T4 polynucleotide kinase
GC-010-0500
Availability:
500 u
17,50 € excl tax
T4 polynucleotide kinase
GC-010-1000
Availability:
1000 u
35,00 € excl tax
T4 polynucleotide kinase
GC-010-5000
Availability:
5000 u
175,00 € excl tax

DESCRIPTION

Isolated from a recombinant E. coli strain this enzyme catalyzes the transfer of the terminal phosphate group of ATP to 5'-OH ends of DNA. T4 polynucleotide kinase is suited for labelling of 5'-OH ends of DNA with ã32P-ATP.


CONCENTRATION

10 units/µl


UNIT DEFINITION

One unit is the amount of enzyme that incorporates 1 nmol of acid-insoluble 32P in 30 minutes at 37ºC.


STORAGE BUFFER

10 mM K-phosphate buffer pH 7.0; 100 mM NaCl; 0.5 mM EDTA; 1 mM DTT; 0.01% Tween 20; 50% glycerol (v/v)


STORAGE TEMPERATURE

Store T4 polynucleotide kinase below 0ºC, preferably at -20ºC, in a constant temperature freezer.


5X REACTION BUFFER

350 mM Tris-HCl pH 7,6; 50 mM MgCl2; 25 mM DTT