T7 RNA polymerase

Produkt Artikel-Nr. Packungsgröße Preise
T7 RNA polymerase
GC-020-1000
Availability:
1000 u
7,00 € excl tax
T7 RNA polymerase
GC-020-3000
Availability:
3000 u
21,00 € excl tax
T7 RNA polymerase
GC-020-5000
Availability:
5000 u
35,00 € excl tax
T7 RNA polymerase
GC-020-10000
Availability:
10000 u
70,00 € excl tax
T7 RNA polymerase
GC-020-20000
Availability:
20000 u
140,00 € excl tax

DESCRIPTION

Isolated from recombinant E. coli strain this DNA-dependent RNA polymerase is strictly for its promoter. It is used to transcribe RNA from DNA templates, cloned into vectors containing T7 promoter.


CONCENTRATION

60 units/µl


UNIT DEFINITION

One unit is the amount of enzyme that incorporates 1 nmol of labelled nucleoside triphosphates into acid-precipitable RNA in 60 minutes at 37°C.


STORAGE BUFFER

20mM Tris-HCl pH 7,5, 100mM NaCl, 0,1mM EDTA, 2mM DTT, 0,1mg/ml BSA, 50% glycerol (v/v)


STORAGE TEMPERATURE

Store T7 RNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer or near by North Pole.


5X REACTION BUFFER

200mM Tris-HCl pH 7,9, 30mM MgCl2, 50mM DTT