E. coli uracil-DNA glycosylase (UDG) is the recombinant form of the enzyme, which catalyzes the release of free uracil from uracil-containing DNA (U-DNA) and creates an alkali-sensitive apyrimidinic site in the DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded U-DNA, but not from oligomers (6 or fewer bases).
- site-directed mutagenesis
- as a probe for protein-DNA interaction studies producing highly labeled oligonucleotide probes
One unit of enzyme activity is the amount that catalyzes the degradation of 1 µg single-stranded uracil-con-taining DNA at 37°C in 60 minutes.
20 mM Tris-HCl pH 8.0; 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 0.1 mg/ml BSA; 50% glycerol
Storage temperature -20°C
10X REACTION BUFFER
500 mM Tricine pH 8.5, 10 mM MgCl2 or buffer for PCR or RT-PCR protocol
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.