Uracil-DNA glycosylase

Produkt Artikel-Nr. Packungsgröße Preise
Uracil-DNA glycosylase
GC-038-0100
Manufacturer part number:
Availability:
100 u
20,00 € excl tax
Uracil-DNA glycosylase
GC-038-0250
Manufacturer part number:
Availability:
250 u
50,00 € excl tax
Uracil-DNA glycosylase
GC-038-0500
Manufacturer part number:
Availability:
500 u
100,00 € excl tax
Uracil-DNA glycosylase
GC-038-1000
Manufacturer part number:
Availability:
1000 u
200,00 € excl tax
Uracil-DNA glycosylase
GC-038-5000
Manufacturer part number:
Availability:
5000 u
1.000,00 € excl tax

DESCRIPTION

E. coli uracil-DNA glycosylase (UDG) is the recombinant form of the enzyme, which catalyzes the release of free uracil from uracil-containing DNA (U-DNA) and creates an alkali-sensitive apyrimidinic site in the DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded U-DNA, but not from oligomers (6 or fewer bases).


APPLICATION

  • PCR
  • RT-PCR
  • site-directed mutagenesis
  • as a probe for protein-DNA interaction studies producing highly labeled oligonucleotide probes


CONCENTRATION

5 units/µl


UNIT DEFINITION

One unit of enzyme activity is the amount that catalyzes the degradation of 1 µg single-stranded uracil-con-taining DNA at 37°C in 60 minutes.


STORAGE BUFFER

20 mM Tris-HCl pH 8.0; 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 0.1 mg/ml BSA; 50% glycerol


STORAGE TEMPERATURE

Storage temperature -20°C


10X REACTION BUFFER

500 mM Tricine pH 8.5, 10 mM MgCl2 or buffer for PCR or RT-PCR protocol


QUALITY CONTROL

Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.